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M94A3306.TXT
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1994-10-25
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Document 3306
DOCN M94A3306
TI Role of natural suppressor glutamine tRNA on growth control of
retroviruses.
DT 9412
AU Kuchino Y; Muller WE; Odawara T; Yoshikura H; Biophys. Div., National
Cancer Center Res. Inst., Tokyo, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0041). Unique
Identifier : AIDSLINE ICA10/94369269
AB Frameshift suppression or nonsense suppression has a crucial role in
expression regulation of retrovirus genomic informations. Recently, we
isolated a natural suppressor tRNA from human and rodent cells, which
recognizes the UAG amber codon as a glutamine codon in an in vitro
translation system prepared from a rabbit reticulocyte lysate.
Interestingly, the increase of the glutamine tRNA was dependent on
retrovirus infection and subsequent vegetative proliferation of the
virus such as MoMuLV and HIV-1. Therefore, it is imoportant to
understand the molecular mechanism of expression regulation of the
glutamine tRNA in mammalian cells for developing of the inhibition
method of virus proliferation. Our recent investigations indicated that
the mammalian gene encoding the suppressor glutamine tRNA commonly
contained a unique signal sequence in the 5'-flanking region of the
gene. In addition, we found that the binding of a nuclear protein to the
sequence inhibited the transcription activity of the tRNA gene in vitro.
We would like to discuss the transcriptional regulation of the
suppressor glutamine tRNA gene in retrovirus infected cells.
DE Animal Base Sequence Codon/GENETICS *Genes, Suppressor
Glutamine/*GENETICS Human HIV-1/GROWTH &
DEVELOPMENT/GENETICS/PHYSIOLOGY In Vitro Moloney Leukemia Virus/GROWTH
& DEVELOPMENT/GENETICS/PHYSIOLOGY Retroviridae/*GROWTH &
DEVELOPMENT/*GENETICS/PHYSIOLOGY Retroviridae Infections/MICROBIOLOGY
RNA, Transfer/*GENETICS Transcription, Genetic Virus
Replication/GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).